Journal: Journal of Biological Chemistry

Article Title: The role of striated muscle Pik3r1 in glucose and protein metabolism following chronic glucocorticoid exposure

doi: 10.1016/j.jbc.2021.100395

Figure Lengend Snippet: Figure 6. Comparing signaling pathways that regulate protein synthesis in gastrocnemius muscle of WT and MKO mice. WT and MKO mice were treated with or without DEX (10 mg/kg body weight) for 1 week. At the end of treatment, mice were IP injected with insulin (1 unit per kg body weight) or PBS for 10 min, and gastrocnemius muscle was isolated and processed for ELISA to monitor the phosphorylation status of (A) p70S6K at Thr389, (B) 4E-BP1 at Thr37/Ser46 and (C) eIF2α at Ser52. n = 3 to 6, significance was denoted as *p ≤0.05. Error bars represent the SD. DEX, dexamethasone.

Article Snippet: All steps afterward were followed according to manufacturer’s instructions. eIF2α and phospho-eIF2α ELISA Relative total eIF2α and phosphorylated eIF2α levels were measured using the PathScan Phospho-eIF2α (Ser51) and total eIF2α Sandwich ELISA kits (Cell Signaling Technology, Catalog #7286 and Catalog #7952.)

Techniques: Protein-Protein interactions, Injection, Isolation, Enzyme-linked Immunosorbent Assay, Phospho-proteomics